Tln1^tm1.2Geno
Targeted Allele Detail

Summary

• Symbol: Tln1^tm1.2Geno
• Name: talin 1; targeted mutation 1.2, Genoway
• MGI ID: MGI:6281422
• Synonyms: Tln1^E1770A
• Gene: Tln1 Location: Chr4:43531513-43562583 bp, - strand Genetic Position: Chr4, 23.05 cM
• Alliance: Tln1^tm1.2Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271017
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A minigene consisting of the entire wild-type sequence from exons 41 to the terminal coding exon 59, followed by a polyadenylation sequence was used in the targeting vector. The minigene and a FRT-flanked neo-selection cassette were flanked with loxP sites and placed upstream of a mutated exon 42 sequence that substitutes the glutamate at amino acid 1770 to an alanine. Flp-mediated recombination removed the neomycin cassette. Germline cre-mediated recombination removed the minigene resulting in splicing on to the mutated exon 42 leading to transcription of the E1770A mutant. This mutation is predicted to abrogate binding with the FERM domain and to block autoinhibition. (J:271017)

Expression

In Mice Carrying this Mutation:

9 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tln1 Mutation: 147 strains or lines available

References

• Original: J:271017 Haage A, et al., Talin Autoinhibition Regulates Cell-ECM Adhesion Dynamics and Wound Healing In Vivo. Cell Rep. 2018 Nov 27;25(9):2401-2416.e5
• All: 2 reference(s)