Sft2d2^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sft2d2^em1(IMPC)J
• Name: SFT2 domain containing 2; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6283505
• Gene: Sft2d2 Location: Chr1:165001906-165022007 bp, - strand Genetic Position: Chr1, 72.51 cM
• Alliance: Sft2d2^em1(IMPC)J page
• IMPC: Sft2d2 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ATATCTACCGCGTTTGAGGG and TGTGTTAGAGATGGTCTGAA, which resulted in a 521 bp deletion beginning at Chromosome 1 position 165,187,898 bp and ending after 165,188,418 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000229846 and ENSMUSE00000229842 (exons 2 and 3) and 348 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 21 and early truncation 15 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Sft2d2 Mutation: 11 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 2 reference(s)