Zfp296^tm1Miya
Targeted Allele Detail

Summary

• Symbol: Zfp296^tm1Miya
• Name: zinc finger protein 296; targeted mutation 1, Jun-ichi Miyazaki
• MGI ID: MGI:6283680
• Synonyms: Zfp296^-
• Gene: Zfp296 Location: Chr7:19311212-19314581 bp, + strand Genetic Position: Chr7, 9.93 cM
• Alliance: Zfp296^tm1Miya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255491
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert an IRES-beta-geo-pA cassette into exon 3 which encodes all of the zinc-finger domains. Western blot analysis confirmed the absence of protein expression in the adult testis of homozygous mutant mice. (J:255491)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zfp296 Mutation: 15 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:255491 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:255491 Matsuura T, et al., Zfp296 negatively regulates H3K9 methylation in embryonic development as a component of heterochromatin. Sci Rep. 2017 Sep 29;7(1):12462
• All: 1 reference(s)