Kirrel2^{tm2b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kirrel2^{tm2b(KOMP)Wtsi}
• Name: kirre like nephrin family adhesion molecule 2; targeted mutation 2b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6285518
• Synonyms: Kirrel2^-
• Gene: Kirrel2 Location: Chr7:30146959-30157115 bp, - strand Genetic Position: Chr7, 18.36 cM
• Alliance: Kirrel2^{tm2b(KOMP)Wtsi} page
• IMPC: Kirrel2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272414
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30154397 of Chromosome 7 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 30155529. Exons 3 and 4 are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm2a allele to remove the neo selection cassette and loxP-flanked exons 3 and 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. In situ hybridization and immunohistochemical analyses confirmed the absence of transcript and protein expression in the vomeronasal organ (VNO) and on vomeronasal sensory neuron (VSN) axons of homozygous mutant mice, respectively. (J:272414)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kirrel2 Mutation: 25 strains or lines available

References

• Original: J:272414 Brignall AC, et al., Loss of Kirrel family members alters glomerular structure and synapse numbers in the accessory olfactory bulb. Brain Struct Funct. 2018 Jan;223(1):307-319
• All: 3 reference(s)