Kirrel2^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kirrel2^{tm2c(KOMP)Wtsi}
• Name: kirre like nephrin family adhesion molecule 2; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6285519
• Synonyms: Kirrel2^lox
• Gene: Kirrel2 Location: Chr7:30146959-30157115 bp, - strand Genetic Position: Chr7, 18.36 cM
• Alliance: Kirrel2^{tm2c(KOMP)Wtsi} page
• IMPC: Kirrel2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272414
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30154397 of Chromosome 7 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 30155529. Exons 3 and 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving exons 3 and 4 flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:272414)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kirrel2 Mutation: 25 strains or lines available

References

• Original: J:272414 Brignall AC, et al., Loss of Kirrel family members alters glomerular structure and synapse numbers in the accessory olfactory bulb. Brain Struct Funct. 2018 Jan;223(1):307-319
• All: 1 reference(s)