Pcdhg^em8Rwb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pcdhg^em8Rwb
• Name: protocadherin gamma cluster; endonuclease-mediated mutation 8, Robert W Burgess
• MGI ID: MGI:6285783
• Synonyms: 1R1
• Gene: Pcdhg Location: unknown Genetic Position: Chr18, Syntenic

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s), Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated through shotgun CRISPR/Cas9 editing using pooled sgRNAs that targeted each variable exon of the Pcdhg cluster and this allele was found to have a frameshift indel of exon C5 and a large rearrangement or deletion that encompasses almost all other variable exons from A1 through C3, leaving only exon C4 intact. (J:285314)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcdhg Mutation: 13 strains or lines available

References

• Original: J:285314 Garrett AM, et al., CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4. PLoS Genet. 2019 Dec;15(12):e1008554
• All: 3 reference(s)