Pcdhg^em35Rwb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pcdhg^em35Rwb
• Name: protocadherin gamma cluster; endonuclease-mediated mutation 35, Robert W Burgess
• MGI ID: MGI:6285828
• Synonyms: 3R2
• Gene: Pcdhg Location: unknown Genetic Position: Chr18, Syntenic

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: This allele was generated through shotgun CRISPR/Cas9 editing using pooled sgRNAs that targeted each variable exon of the Pcdhg cluster and this allele was found to have small frameshift mutations disrupting the variable exons A12 and C5, a 9bp in-frame deletion in variable exon C4, which deletes amino acids 27-29 in the signal peptide, and a large 94 kb deletion spanning from variable exon A1 into A11, which leaves un-mutated only variable exons B8 and C3. (J:285314)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcdhg Mutation: 13 strains or lines available

References

• Original: J:285314 Garrett AM, et al., CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4. PLoS Genet. 2019 Dec;15(12):e1008554
• All: 2 reference(s)