Del(6Gimap5-Gimap3)1Yout
Targeted Allele Detail

Summary

• Symbol: Del(6Gimap5-Gimap3)1Yout
• Name: deletion, Chr 6, Yousuke Takahama
• MGI ID: MGI:6286400
• Synonyms: Gimap3^- Gimap5^-
• Gene: Del(6Gimap5-Gimap3)1Yout Location: Chr6:48723131-48747785 bp Genetic Position: Chr6, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209926
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Mutation details: This allele was created by crossing mice carrying the Gimap5^tm1Yout allele with mice carrying the Gimap3^tm1.1Yout and Tg(CAG-cre)13Miya alleles. The Gimap5^tm1Yout allele carries two loxP sites flanking the neo cassette inserted into, and replacing the first 35 coding nucleotides of, exon 2. The Gimap3^tm1.1Yout allele, which is in the opposite genomic orientation, carries a single loxP site in the 3' UTR of the gene (as a consequence of the deletion of the floxed CDS) in the same orientation as the Gimap5^tm1Yout loxP sites. The presence of the Tg(CAG-cre)13Miya allele results in cre-mediated recombination between the loxP sites, thus deleting the entire coding region of Gimap5 and the intergenic sequence between the two genes. This creates a single deletion allele representing a Gimap5 Gimap3 double knockout. (J:209926)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(6Gimap5-Gimap3)1Yout Mutation: 0 strains or lines available

References

• Original: J:209926 Yano K, et al., Gimap3 and Gimap5 cooperate to maintain T-cell numbers in the mouse. Eur J Immunol. 2014 Feb;44(2):561-72
• All: 1 reference(s)