Cenpf^em2Bko
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cenpf^em2Bko
• Name: centromere protein F; endonuclease-mediated mutation 2, Benoit Kornmann
• MGI ID: MGI:6287440
• Synonyms: cenpf^{[p.G2871fsX33]BKL}, MUT1
• Gene: Cenpf Location: Chr1:189372803-189420283 bp, - strand Genetic Position: Chr1, 95.03 cM
• Alliance: Cenpf^em2Bko page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 2 bp deletion with a 4 bp insertion at the very beginning of exon 17 resulting in a frameshift starting from residue G2871 which is expected to result in truncation of the last two exons which encode the C-terminal microtubule-binding domain and the farnesylation motif. Western blot analysis with an antibody against the C-terminus confirmed that the C-terminus is missing. Western blot with an antibody against an epitope upstream of the truncation show reduced protein levels. However, quantitative RT-PCR did not detect differences in mRNA levels, indicating that protein is destabilized. (J:273098)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cenpf Mutation: 109 strains or lines available

References

• Original: J:273098 Peterka M, et al., Miro-dependent mitochondrial pool of CENP-F and its farnesylated C-terminal domain are dispensable for normal development in mice. PLoS Genet. 2019 Mar;15(3):e1008050
• All: 1 reference(s)