Ccnb3^em1Sky
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ccnb3^em1Sky
• Name: cyclin B3; endonuclease-mediated mutation 1, Scott Keeney
• MGI ID: MGI:6287726
• Synonyms: Ccnb3^-
• Gene: Ccnb3 Location: ChrX:6845891-6907858 bp, - strand Genetic Position: ChrX, 3.13 cM
• Alliance: Ccnb3^em1Sky page

Mutation origin

• Strain of Origin: (CBA/J x C57BL/6J)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 14 bp deletion at the 3' end of the 2.7-kb long exon 7, causing a frameshift and premature stop codon upstream of the cyclin box in exons 9-13. Exon 7 was targeted by guide RNA C40-TGAACTTGGCATGATAGCAC. Immunoprecipitation and Western blot analysis yielded no signal for either full-length or the predicted truncation product in adult testes extracts indicating a null or severe loss-of-function allele. (J:273304)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccnb3 Mutation: 6 strains or lines available

References

• Original: J:273304 Karasu ME, et al., Cyclin B3 promotes anaphase I onset in oocyte meiosis. J Cell Biol. 2019 Apr 1;218(4):1265-1281
• All: 3 reference(s)