Spatc1^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spatc1^em1(IMPC)J
• Name: spermatogenesis and centriole associated 1; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6293902
• Gene: Spatc1 Location: Chr15:76152289-76176772 bp, + strand Genetic Position: Chr15, 35.63 cM, cytoband E1
• Alliance: Spatc1^em1(IMPC)J page
• IMPC: Spatc1 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CCAACCCCACACCAGAAGAG and CTACCCCTTTGCTAAGAACA, which resulted in a 9510 bp deletion beginning at Chromosome 15 position 76,283,317 bp and ending after 76,292,826 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000498536-ENSMUSE00000479099 (exons 2-5) and 8193 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 69 and early truncation 29 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Spatc1 Mutation: 20 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)