Scfd2^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Scfd2^em1(IMPC)J
• Name: Sec1 family domain containing 2; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6293920
• Gene: Scfd2 Location: Chr5:74365477-74692420 bp, - strand Genetic Position: Chr5, 39.44 cM
• Alliance: Scfd2^em1(IMPC)J page
• IMPC: Scfd2 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTTCTGGGCTGCCTCCGCAG and ATCAAGTTAATACTTCCCAG, which resulted in a 220 bp deletion beginning at Chromosome 5 position 74,482,133 bp and ending after 74,482,352 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000227750 (exon 3) and 92 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 335 and early truncation 22 amino acids later. There is a single bp (T) insertion at the deletion site. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Scfd2 Mutation: 42 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)