Rab31^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rab31^em1(IMPC)J
• Name: RAB31, member RAS oncogene family; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6294099
• Gene: Rab31 Location: Chr17:65958724-66079747 bp, - strand Genetic Position: Chr17, 35.03 cM
• Alliance: Rab31^em1(IMPC)J page
• IMPC: Rab31 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTGGCTTTCTAGGGACAAAA and GGAGTTTCGATGATGCAGAA, which resulted in a 142 bp deletion beginning at Chromosome 17 position 65,717,444 bp and ending after 65,717,585 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000657070 (exon 3) and 60 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 40 and early truncation 29 amino acids later. There is a 5 bp insertion (TAAAG) at the deletion site. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rab31 Mutation: 33 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)