Trim68^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Trim68^em1(IMPC)J
• Name: tripartite motif-containing 68; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6294128
• Gene: Trim68 Location: Chr7:102326789-102336534 bp, - strand Genetic Position: Chr7, 54.8 cM
• Alliance: Trim68^em1(IMPC)J page
• IMPC: Trim68 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTTCTTTCACTAATGTAAGA and TTTTAGGGTAAGGGTCACGT, which resulted in a 2368 bp deletion beginning at Chromosome 7 position 102,679,556 bp and ending after 102,681,923 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000366315 and ENSMUSE00000401581 (exons 4 and 5) and 2084 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 174 and early truncation 66 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Trim68 Mutation: 20 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)