Piezo2^em2(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Piezo2^em2(IMPC)J
• Name: piezo-type mechanosensitive ion channel component 2; endonuclease-mediated mutation 2, Jackson
• MGI ID: MGI:6294748
• Gene: Piezo2 Location: Chr18:63143284-63520254 bp, - strand Genetic Position: Chr18, 35.95 cM, cytoband D3
• Alliance: Piezo2^em2(IMPC)J page
• IMPC: Piezo2 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TGGGGCTCATTTCAACCTAA and CCTTGACGTTGGAAAGCCAA, which resulted in a 417 bp deletion beginning at Chromosome 18 position 63,030,224 bp and ending after 63,030,640 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001304790 (exon 42) and 164 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 2106 and early truncation 15 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Piezo2 Mutation: 123 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)