Abca8a^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Abca8a^em1(IMPC)J
• Name: ATP-binding cassette, sub-family A member 8a; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6294772
• Gene: Abca8a Location: Chr11:109916460-109986804 bp, - strand Genetic Position: Chr11, 73.07 cM
• Alliance: Abca8a^em1(IMPC)J page
• IMPC: Abca8a gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ACAGAGTCTTTCCTCTAAGT and GCTTACCCTATCACTACGTA, which resulted in a 1110 bp deletion beginning at Chromosome 11 position 110,071,149 bp and ending after 110,072,258 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000342464 and ENSMUSE00001068496 (exons 11 and 12) and 940 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 478 and early truncation 5 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Abca8a Mutation: 103 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)