Cdk5rap3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cdk5rap3^{tm1c(EUCOMM)Hmgu}
• Name: CDK5 regulatory subunit associated protein 3; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6294784
• Gene: Cdk5rap3 Location: Chr11:96798252-96807322 bp, - strand Genetic Position: Chr11, 60.16 cM
• Alliance: Cdk5rap3^{tm1c(EUCOMM)Hmgu} page
• IMPC: Cdk5rap3 gene page

Mutation origin

• Mutant Cell Line: HEPD0516_2_A06
• Germline Transmission: Earliest citation of germline transmission: J:272566
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 96800537 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 96803660. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:272566)

Expression

In Mice Carrying this Mutation:	81 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk5rap3 Mutation: 20 strains or lines available

References

• Original: J:272566 Yang R, et al., CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development. Development. 2019 Jan 25;146(2):dev169235
• All: 3 reference(s)