Nucks1^tm1.2Vter
Targeted Allele Detail

Summary

• Symbol: Nucks1^tm1.2Vter
• Name: nuclear casein kinase and cyclin-dependent kinase substrate 1; targeted mutation 1.2, Vinay Tergaonkar
• MGI ID: MGI:6294929
• Synonyms: Nucks1^-
• Gene: Nucks1 Location: Chr1:131838272-131864059 bp, + strand Genetic Position: Chr1, 57.22 cM
• Alliance: Nucks1^tm1.2Vter page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211777
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 4. The neo cassette was removed through subsequent flp-mediated recombination. Exon 4 was deleted through subsequent cre-mediated recombination. Western blot analysis showed an absent of expression in mouse embryonic fibroblasts, liver, and epididymal adipose tissues. (J:211777)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nucks1 Mutation: 68 strains or lines available

References

• Original: J:211777 Qiu B, et al., NUCKS Is a Positive Transcriptional Regulator of Insulin Signaling. Cell Rep. 2014 Jun 26;7(6):1876-86
• All: 1 reference(s)