Eif3etm1Fshb
Targeted Allele Detail
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| Symbol: |
Eif3etm1Fshb |
| Name: |
eukaryotic translation initiation factor 3, subunit E; targeted mutation 1, Futoshi Shibasaki |
| MGI ID: |
MGI:6295473 |
| Gene: |
Eif3e Location: Chr15:43113454-43146115 bp, - strand Genetic Position: Chr15, 16.73 cM
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| Alliance: |
Eif3etm1Fshb page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:265010
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| Parent Cell Line: |
RENKA (ES Cell)
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| Strain of Origin: |
C57BL/6N
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| Allele Type: |
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Targeted (Conditional ready, Null/knockout) |
| Mutation: |
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Insertion
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Mutation details: The EUCOMM targeting vector PG00188_Y_2_D09 in which the L1L2_Bact_P cassette was inserted at position 43275745 of Chromosome 15 upstream of the critical exon 3 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 43274946. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created.
(J:265010)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Eif3e Mutation: |
33 strains or lines available
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| Original: |
J:265010 Sadato D, et al., Eukaryotic translation initiation factor 3 (eIF3) subunit e is essential for embryonic development and cell proliferation. FEBS Open Bio. 2018 Aug;8(8):1188-1201 |
| All: |
1 reference(s) |
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Analysis Tools
