Fgf10^{tm2.1(cre/ERT2)Sbel}
Targeted Allele Detail

Summary

• Symbol: Fgf10^{tm2.1(cre/ERT2)Sbel}
• Name: fibroblast growth factor 10; targeted mutation 2.1, Saverio Bellusci
• MGI ID: MGI:6296741
• Synonyms: Fgf10Cre-ERT2, Fgf10Ki-v2, Fgf10^{tm2(eYFP-Cre/ERT2)Sbel}
• Gene: Fgf10 Location: Chr13:118851199-118928651 bp, + strand Genetic Position: Chr13, 67.14 cM, cytoband A3-A4
• Alliance: Fgf10^{tm2.1(cre/ERT2)Sbel} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308431
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Fgf10^{tm2.1(cre/ERT2)Sbel} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Fgf10 (MGI:1099809)

• Mutation details: An EGFP gene and inducible cre/ERT2 fusion were inserted into the 3' UTR immediately downstream of the stop codon of the exon 3. The self-cleavage LF2A and T2A sequences will lead to 3 distinct proteins. Flp-mediated recombination removed the selection cassette. The homozygous embryos suffered from lung and limb agenesis and the analysis of Fgf10 expression by qPCR indicated a drastic reduction in Fgf10 expression. It is a loss-of-function allele. (J:308431)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Fgf10 (mouse) Summary of all recombinase alleles driven by Fgf10. Comparative matrix view of recombinase activities Alliance mouse Fgf10 gene page

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf10 Mutation: 33 strains or lines available

Notes

S3 = C57BL/6NCrl

References

• Original: J:308431 Chu X, et al., Validation of a Novel Fgf10 (Cre-ERT2) Knock-in Mouse Line Targeting FGF10(Pos) Cells Postnatally. Front Cell Dev Biol. 2021;9:671841
• All: 1 reference(s)