Bdh1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Bdh1^{tm1c(EUCOMM)Wtsi}
• Name: 3-hydroxybutyrate dehydrogenase, type 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6297074
• Gene: Bdh1 Location: Chr16:31241115-31277719 bp, + strand Genetic Position: Chr16, 21.74 cM
• Alliance: Bdh1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Bdh1 gene page

Mutation origin

• Mutant Cell Line: EPD0371_4_A09
• Germline Transmission: Earliest citation of germline transmission: J:274475
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31264966 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 31266725. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:274475)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bdh1 Mutation: 33 strains or lines available

References

• Original: J:274475 Horton JL, et al., The failing heart utilizes 3-hydroxybutyrate as a metabolic stress defense. JCI Insight. 2019 Feb 21;4(4)
• All: 5 reference(s)