Tomm70a^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tomm70a^em1(IMPC)J
• Name: translocase of outer mitochondrial membrane 70A; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6302770
• Synonyms: Tomm70a^-
• Gene: Tomm70a Location: Chr16:56942077-56974893 bp, + strand Genetic Position: Chr16, 34.22 cM
• Alliance: Tomm70a^em1(IMPC)J page
• IMPC: Tomm70a gene page

Tomm70a^em1(IMPC)J/Tomm70a^em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E7.5 but not at E9.5. Embryos are smaller and form a rudimentary egg cylinder but no primitive streak or hallmarks of gastrulation are seen at E7.5.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTATTGCCTAGTGTTCGGGA and AGAGAACAAAGTCTCAACCG, which resulted in a 496 bp deletion beginning at Chromosome 16 position 57,134,423 bp and ending after 57,134,918 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000130105 (exon 3) and 369 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 169 and early truncation 7 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tomm70a Mutation: 40 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 4 reference(s)