Pcnx4^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pcnx4^em1(IMPC)J
• Name: pecanex homolog 4; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6305217
• Gene: Pcnx4 Location: Chr12:72583157-72626893 bp, + strand Genetic Position: Chr12, 30.2 cM
• Alliance: Pcnx4^em1(IMPC)J page
• IMPC: Pcnx4 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTATTTACCAAGATAAACTG and CCACAGTTACACTCAGCCAC, which resulted in a 198 bp deletion beginning at Chromosome 12 position 72,556,521 bp and ending after 72,556,718 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000437603 (exon 5) and 97 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 464 and early truncation 32 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pcnx4 Mutation: 55 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)