Dbh^{tm3.2(cre)Pjen}
Targeted Allele Detail

Summary

• Symbol: Dbh^{tm3.2(cre)Pjen}
• Name: dopamine beta hydroxylase; targeted mutation 3.2, Patricia Jensen
• MGI ID: MGI:6305870
• Synonyms: Dbh^cre
• Gene: Dbh Location: Chr2:27055519-27073216 bp, + strand Genetic Position: Chr2, 19.29 cM
• Alliance: Dbh^{tm3.2(cre)Pjen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:285301
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Dbh^{tm3.2(cre)Pjen} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dbh (MGI:94864)

• Mutation details: A rox-flanked transcriptional stop cassette, cre cDNA, rabbit beta-globin polyadenylation cassette, and attB/attP-flanked neomycin resistance cassette were inserted into the Dbh locus, immediately following the start codon by homologous recombination. A recombinant clone was transiently transfected with pPGKPhiC31obpa to excise the neomycin resistance cassette before cells were injected into blastocysts to produce chimeric mice. Following germline transmission, heterozygotes were crossed with Dre expressing mice to remove the rox-flanked stop cassette. (J:285301)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dbh (mouse) Summary of all recombinase alleles driven by Dbh. Comparative matrix view of recombinase activities Alliance mouse Dbh gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dbh Mutation: 17 strains or lines available

References

• Original: J:285301 Tillage RP, et al., Elimination of galanin synthesis in noradrenergic neurons reduces galanin in select brain areas and promotes active coping behaviors. Brain Struct Funct. 2020;225(2):785-803
• All: 7 reference(s)