Cela2a^tm1Bdr
Targeted Allele Detail

Summary

• Symbol: Cela2a^tm1Bdr
• Name: chymotrypsin-like elastase family, member 2A; targeted mutation 1, Michael Bader
• MGI ID: MGI:6313718
• Synonyms: ELA-2KO
• Gene: Cela2a Location: Chr4:141542265-141553316 bp, - strand Genetic Position: Chr4, 74.75 cM
• Alliance: Cela2a^tm1Bdr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275582
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The transgene construct, plasmid PRPGS00048_A_H09 (KOMP project), was electroporated into ES cells. Only clone 2B9 showed proper recombination and was chosen to establish the mouse line. The allele comprises the following elements: an FRT site, a gene trap cassette (mouse En2 splice acceptor, IRES, LacZ gene, SV40 poly(A) signal sequences), a loxP site, a neomycin resistance gene cassette, a second loxP site and a aecond FRT site in intron 2, and a third loxP site in intron 5. (J:275582)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cela2a Mutation: 16 strains or lines available

References

• Original: J:275582 Becari C, et al., Elastase-2, a Tissue Alternative Pathway for Angiotensin II Generation, Plays a Role in Circulatory Sympathovagal Balance in Mice. Front Physiol. 2017;8:170
• All: 1 reference(s)