Zc3h7b^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Zc3h7b^em1(IMPC)J
• Name: zinc finger CCCH type containing 7B; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6314204
• Gene: Zc3h7b Location: Chr15:81629299-81680470 bp, + strand Genetic Position: Chr15, 38.23 cM
• Alliance: Zc3h7b^em1(IMPC)J page
• IMPC: Zc3h7b gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GAGTCTGCTCAGTTAGTCAG and TCCAACCCCCTACCCTAGCC, which resulted in a 289 bp deletion beginning at Chromosome 15 position 81,768,613 bp and ending after 81,768,901 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000253367 (exon 3) and 255 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 18 and early truncation 33 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Zc3h7b Mutation: 61 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)