Fam193a^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fam193a^em1(IMPC)J
• Name: family with sequence homology 193, member A; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6314220
• Gene: Fam193a Location: Chr5:34527295-34643800 bp, + strand Genetic Position: Chr5, 17.88 cM
• Alliance: Fam193a^em1(IMPC)J page
• IMPC: Fam193a gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCTAGGTGCACAAATCCCAT and TTATGTTCACCTCAGCAGCC, which resulted in a 383 bp deletion beginning at Chromosome 5 position 34,426,149 bp and ending after 34,426,531 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001144988 (exon 5) and 148 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 263 and early truncation 4 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Fam193a Mutation: 68 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)