Tbc1d21^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tbc1d21^em1(IMPC)J
• Name: TBC1 domain family, member 21; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6314798
• Gene: Tbc1d21 Location: Chr9:58266987-58277738 bp, - strand Genetic Position: Chr9, 31.7 cM, cytoband C
• Alliance: Tbc1d21^em1(IMPC)J page
• IMPC: Tbc1d21 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCCTAGTCATTTCCAGGCAT and GAGCAAGCGTTCCCAACATG, which resulted in a 519 bp deletion beginning at Chromosome 9 position 58,363,515 bp and ending after 58,364,033 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000253955 (exon 4) and 424 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 90 and early truncation 8 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tbc1d21 Mutation: 24 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)