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B3glcttm1d(KOMP)Wtsi
Targeted Allele Detail
Summary
Symbol: B3glcttm1d(KOMP)Wtsi
Name: beta-3-glucosyltransferase; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID: MGI:6315841
Synonyms: B3glct-delta4
Gene: B3glct  Location: Chr5:149601695-149686064 bp, + strand  Genetic Position: Chr5, 89.18 cM
Alliance: B3glcttm1d(KOMP)Wtsi page
IMPC: B3glct gene page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:286130
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 149632305 of Chromosome 5 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 149633181. The critical exon 4 is thus flanked by loxP sites.A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon 4. The knockout allele was created by deleting the cirtical exon 4 through subsequent cre-mediated recombination. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:286130)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any B3glct Mutation:  48 strains or lines available
References
Original:  J:286130 Holdener BC, et al., ADAMTS9 and ADAMTS20 are differentially affected by loss of B3GLCT in mouse model of Peters plus syndrome. Hum Mol Genet. 2019 Dec 15;28(24):4053-4066
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory