Spag6^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spag6^em1(IMPC)J
• Name: sperm associated antigen 6; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6316614
• Gene: Spag6 Location: Chr2:18698808-18754561 bp, + strand Genetic Position: Chr2, 12.9 cM
• Alliance: Spag6^em1(IMPC)J page
• IMPC: Spag6 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTAGGTTAGTTACTAACCAG and AGGGAATCTGAAGGAGACGG, which resulted in a 533 bp deletion beginning at Chromosome 2 position 18,715,493 bp and ending after 18,716,025 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000605260 (exon 4) and 349 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 96 and early truncation 14 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Spag6 Mutation: 36 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)