Ttc29^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ttc29^em1(IMPC)J
• Name: tetratricopeptide repeat domain 29; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6317331
• Gene: Ttc29 Location: Chr8:78939926-79120955 bp, + strand Genetic Position: Chr8, 36.86 cM
• Alliance: Ttc29^em1(IMPC)J page
• IMPC: Ttc29 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences AGCCATTTTAGACCAGAGAG and GTTCAACCTATTCCACTGAG, which resulted in a 383 bp deletion beginning at Chromosome 8 position 78,246,056 bp and ending after 78,246,438 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000249377 (exon 6) and 159 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 59 and early truncation 2 amino acids later. There is a 1 bp (T) insertion at the deletion site. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ttc29 Mutation: 29 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)