Ndufa10^{tm1e.1(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ndufa10^{tm1e.1(EUCOMM)Hmgu}
• Name: NADH:ubiquinone oxidoreductase subunit A10; targeted mutation 1e.1, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6317360
• Gene: Ndufa10 Location: Chr1:92367208-92401547 bp, - strand Genetic Position: Chr1, 46.4 cM, cytoband D
• Alliance: Ndufa10^{tm1e.1(EUCOMM)Hmgu} page
• IMPC: Ndufa10 gene page

Mutation origin

• Mutant Cell Line: HEPD0520_4_F03
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 92399163 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre-mediated excision of the parental allele resulted in the removal of the promoter-driven neomycin selection cassette leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:82809)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ndufa10 Mutation: 25 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 3 reference(s)