Dennd5b^em1Smgo
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dennd5b^em1Smgo
• Name: DENN domain containing 5B; endonuclease-mediated mutation 1, Scott M Gordon
• MGI ID: MGI:6317361
• Synonyms: Dennd5b^-
• Gene: Dennd5b Location: Chr6:148889569-149003178 bp, - strand Genetic Position: Chr6, 78.71 cM
• Alliance: Dennd5b^em1Smgo page

Mutation origin

• Strain of Origin: (C57BL/6 x CBA)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Using custom zinc finger nucleases, exons in the 5' one-third of the gene were targeted. ZFN RNA was microinjected into pronuclei of fertilized B6CBAF1 mouse embryos. This led to the establishment of a line with a biallelic 19 bp deletion (GGGTCTTCAGTCAAAAGAA) in exon 4 in the DENN domain of the encoded peptide. (J:276600)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dennd5b Mutation: 202 strains or lines available

References

• Original: J:276600 Gordon SM, et al., DENND5B Regulates Intestinal Triglyceride Absorption and Body Mass. Sci Rep. 2019 Mar 5;9(1):3597
• All: 1 reference(s)