Opalin^tm1Tfr
Targeted Allele Detail

Summary

• Symbol: Opalin^tm1Tfr
• Name: oligodendrocytic myelin paranodal and inner loop protein; targeted mutation 1, Teiichi Furuichi
• MGI ID: MGI:6322964
• Synonyms: Neo plus allele
• Gene: Opalin Location: Chr19:41050398-41065552 bp, - strand Genetic Position: Chr19, 34.36 cM
• Alliance: Opalin^tm1Tfr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:257142
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 - 6 were replaced by an FRT-flanked Pgk1 gene promoter-driven neomycin resistance gene (Pgk-neo) cassette. Western blot analysis revealed the complete absence of protein expression from myelin fractions of homozygous mutant brains at weaning, young adult and adult stages. Immunohistochemical analysis confirmed the absence of protein expression in the brains at 16 weeks of age. Expression of other tested major myelin components is similar to that in wild-type controls. (J:257142)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Opalin Mutation: 9 strains or lines available

References

• Original: J:257142 Yoshikawa F, et al., Mammalian-Specific Central Myelin Protein Opalin Is Redundant for Normal Myelination: Structural and Behavioral Assessments. PLoS One. 2016;11(11):e0166732
• All: 1 reference(s)