Cetn1^tm1.1Wbae
Targeted Allele Detail

Summary

• Symbol: Cetn1^tm1.1Wbae
• Name: centrin 1; targeted mutation 1.1, Wolfgang Baehr
• MGI ID: MGI:6324193
• Synonyms: Cetn1^-
• Gene: Cetn1 Location: Chr18:9618419-9619469 bp, - strand Genetic Position: Chr18, 4.91 cM, cytoband A2
• Alliance: Cetn1^tm1.1Wbae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276829
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A single loxP site was inserted 5' to the single coding exon, and a loxP-flanked neomycin selection cassette was inserted 3' to the coding exon, but 5' to the noncoding 3'-UTR exon. Germline deletion of the coding exon and the neomycin cassette was achieved by mating 3loxP mice with an EIIa-Cre line which expresses Cre recombinase under the control of the adenovirus EIIa promoter in mouse embryos. Immunoblot analysis of testes lysates confirmed the absence of protein expression in homozygous mutant males. Immunohistochemistry revealed that protein is undetectable in retina sections and seminiferous tubules. (J:276829)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cetn1 Mutation: 2 strains or lines available

References

• Original: J:276829 Avasthi P, et al., Germline deletion of Cetn1 causes infertility in male mice. J Cell Sci. 2013 Jul 15;126(Pt 14):3204-13
• All: 1 reference(s)