Ly6g^{tm2621(cre)Arte}
Targeted Allele Detail

Summary

• Symbol: Ly6g^{tm2621(cre)Arte}
• Name: lymphocyte antigen 6 family member G; targeted mutation 2621, TaconicArtemis
• MGI ID: MGI:6330745
• Synonyms: Ly6g^{tm1.1(cre)Gunz}, Ly6g^{tm2621(cre,-tdTomato)Arte}, Ly6g(tm2621(CretdTomato)Arte
• Gene: Ly6g Location: Chr15:75027110-75030977 bp, + strand Genetic Position: Chr15, 34.29 cM, cytoband E1
• Alliance: Ly6g^{tm2621(cre)Arte} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227159
• Parent Cell Line: ART B6 3.6 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutation: Insertion
• Ly6g^{tm2621(cre)Arte} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ly6g (MGI:109440)

• Mutation details: The Ly6g coding sequence in exon 1 and the splice donor site at the junction of exon 1 and intron 1 were replaced with a cassette containing the open reading frame of Cre recombinase and tdTomato separated by a self-splicing T2A peptide. A human growth hormone polyadenylation signal was inserted at the 3' end of the Cre-T2A-tdTomato sequence in order to prevent downstream transcription of the remaining mouse Ly6g. The FRT flanked positive selection marker (puromycin resistance (PuroR)) was removed after the successful generation of transgenic mice by crossing with Flpe expressing mice. (J:227159)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ly6g (mouse) Summary of all recombinase alleles driven by Ly6g. Comparative matrix view of recombinase activities Alliance mouse Ly6g gene page

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Ly6g^tm2621(cre)Arte/Ly6g^tm2621(cre)Arte	involves: C57BL/6

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
immune system	N
immune system phenotype	N

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ly6g Mutation: 22 strains or lines available

References

• Original: J:227159 Hasenberg A, et al., Catchup: a mouse model for imaging-based tracking and modulation of neutrophil granulocytes. Nat Methods. 2015 May;12(5):445-52
• All: 35 reference(s)