Dmrt3^em1(cre)Kldr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dmrt3^em1(cre)Kldr
• Name: doublesex and mab-3 related transcription factor 3; endonuclease-mediated mutation 1, Klas Kullander
• MGI ID: MGI:6331094
• Synonyms: Dmrt3^Cre
• Gene: Dmrt3 Location: Chr19:25587665-25601285 bp, + strand Genetic Position: Chr19, 20.5 cM
• Alliance: Dmrt3^em1(cre)Kldr page

Mutation origin

• Strain of Origin: B6D2F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Dmrt3^em1(cre)Kldr expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dmrt3 (MGI:2449470)

• Mutation details: Zinc finger nuclease mRNAs designed for the Dmrt3 locus, and a donor vector carrying iCre and 2A peptide sequence were introduced to B6D2F1 zygotes by pronuclear microinjection. The 2A peptide coding sequence allows for cotranslation of the Dmrt3 promoter-driven iCre followed by Dmrt3. (J:273477)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dmrt3 (mouse) Summary of all recombinase alleles driven by Dmrt3. Comparative matrix view of recombinase activities Alliance mouse Dmrt3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dmrt3 Mutation: 12 strains or lines available

References

• Original: J:273477 Perry S, et al., Characterization of Dmrt3-Derived Neurons Suggest a Role within Locomotor Circuits. J Neurosci. 2019 Mar 6;39(10):1771-1782
• All: 3 reference(s)