Gfra1^{tm4.1(cre/ERT2)Jmi}
Targeted Allele Detail

Summary

• Symbol: Gfra1^{tm4.1(cre/ERT2)Jmi}
• Name: glial cell line derived neurotrophic factor family receptor alpha 1; targeted mutation 4.1, Jeffrey Milbrandt
• MGI ID: MGI:6331276
• Synonyms: GFRa1-CreERT2
• Gene: Gfra1 Location: Chr19:58224036-58444341 bp, - strand Genetic Position: Chr19, 54.18 cM, cytoband D2-D3
• Alliance: Gfra1^{tm4.1(cre/ERT2)Jmi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272383
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intergenic deletion
• Gfra1^{tm4.1(cre/ERT2)Jmi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gfra1 (MGI:1100842)

• Mutation details: A gene cassette composed of CreERT2 cDNA-BGH poly(A) followed by a floxed angiotensin converting enzyme (ACE)-Cre and Tn5-neo cassette were introduced into the second exon of the Gfra1 gene by gene targeting. This insertion deleted 95 nucleotides containing the 5'-UTR, the initiator Met and the signal sequences. Upon germ line transmission of the targeted allele, floxed ACE-Cre and Tn5-neo were selfexcised by ACE-Cre-mediated recombination in ES-cell-derived sperm. (J:272383)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gfra1 (mouse) Summary of all recombinase alleles driven by Gfra1. Comparative matrix view of recombinase activities Alliance mouse Gfra1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gfra1 Mutation: 33 strains or lines available

References

• Original: J:272383 Hara K, et al., Mouse spermatogenic stem cells continually interconvert between equipotent singly isolated and syncytial states. Cell Stem Cell. 2014 May 1;14(5):658-72
• All: 3 reference(s)