Gas2l2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gas2l2^{tm1c(KOMP)Wtsi}
• Name: growth arrest-specific 2 like 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6336196
• Synonyms: Gas2l2^fl
• Gene: Gas2l2 Location: Chr11:83312728-83320281 bp, - strand Genetic Position: Chr11, 50.33 cM
• Alliance: Gas2l2^{tm1c(KOMP)Wtsi} page
• IMPC: Gas2l2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277289
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 83317799 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 83318764. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was then created by flp recombinase expression in mice carrying this allele. (J:277289)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gas2l2 Mutation: 34 strains or lines available

References

• Original: J:277289 Bustamante-Marin XM, et al., Lack of GAS2L2 Causes PCD by Impairing Cilia Orientation and Mucociliary Clearance. Am J Hum Genet. 2019 Feb 7;104(2):229-245
• All: 1 reference(s)