Zfp541^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Zfp541^em1(IMPC)J
• Name: zinc finger protein 541; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6342250
• Gene: Zfp541 Location: Chr7:15795739-15830259 bp, + strand Genetic Position: Chr7, 8.74 cM
• Alliance: Zfp541^em1(IMPC)J page
• IMPC: Zfp541 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CAGTATGATAAGACAAGCAG and GAGTCCACACTGGAGGACAG, which resulted in a 645 bp deletion beginning at Chromosome 7 position 16,076,086 bp and ending after 16,076,730 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000677010 (exon 2) and 404 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 102 and early truncation 43 amino acids later. There is an additional 2 bp deletion (TT) 98 bp before the 645 bp deletion site. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Zfp541 Mutation: 48 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)