Arpp19^{tm1d(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Arpp19^{tm1d(KOMP)Mbp}
• Name: cAMP-regulated phosphoprotein 19; targeted mutation 1d, Mouse Biology Program, UC Davis
• MGI ID: MGI:6342463
• Synonyms: Arpp19^delta
• Gene: Arpp19 Location: Chr9:74944896-74967595 bp, + strand Genetic Position: Chr9, 42.25 cM, cytoband E1
• Alliance: Arpp19^{tm1d(KOMP)Mbp} page
• IMPC: Arpp19 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271162
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 74963458 of Chromosome 9 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 74965535. The critical exon 4 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:271162)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arpp19 Mutation: 33 strains or lines available

References

• Original: J:271162 Hached K, et al., ENSA and ARPP19 differentially control cell cycle progression and development. J Cell Biol. 2019 Feb 4;218(2):541-558
• All: 1 reference(s)