Col1a1^{tm11(tetO-Nup88)Jvd}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm11(tetO-Nup88)Jvd}
• Name: collagen, type I, alpha 1; targeted mutation 11, Jan MA van Deursen
• MGI ID: MGI:6343326
• Synonyms: Nup88^T11
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm11(tetO-Nup88)Jvd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231166
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Epitope tag, Inserted expressed sequence)
• Inducer: doxycycline/tetracyline
• Mutation: Insertion
• Col1a1^{tm11(tetO-Nup88)Jvd} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nup88 (MGI:104900)

• Col1a1^{tm11(tetO-Nup88)Jvd} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: ES cells containing the Gt(ROSA)26Sor^{tm1(rtTA2S-M2)Jae} mutation were retargeted. Cells were injected with a "flip-in plasmid" containing a tetracycline responsive element (TRE or tetO) and mouse Nup88 cDNA sequence with an HA tag and a Flpe-recombinase plasmid to facilitate tetO-Oct4 integration into the 3' UTR region of Col1a1. (J:231166)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

References

• Original: J:231166 Naylor RM, et al., Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy. J Clin Invest. 2016 Feb 1;126(2):543-59
• All: 1 reference(s)