Dnai1^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnai1^em1Osb
• Name: dynein axonemal intermediate chain 1; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6343354
• Synonyms: Dnaic1^{em1(S124A, S127 )Osb}, S124A S127A
• Gene: Dnai1 Location: Chr4:41569775-41638158 bp, + strand Genetic Position: Chr4, 21.75 cM
• Alliance: Dnai1^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 mutagenesis was used to introduce nucleotide changes in exon 6 corresponding to a double amino acid substitution of serine 124 and serine 127 to alanines (S124A and S127A) in the encoded protein. (J:231237)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnai1 Mutation: 47 strains or lines available

References

• Original: J:231237 Young SA, et al., CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis. Int J Mol Sci. 2015;16(10):24732-50
• All: 1 reference(s)