Dnai3^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnai3^em1Osb
• Name: dynein axonemal intermediate chain 3; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6343372
• Synonyms: Wdr63^-472
• Gene: Dnai3 Location: Chr3:145746281-145813855 bp, - strand Genetic Position: Chr3, 71.03 cM
• Alliance: Dnai3^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mutagenesis induced a 472 bp deletion spanning parts of exons 2 and 3. This deletion results in a frameshift mutation at codon 10 and the presence of a stop codon 12 codons later causing a truncation of the encoded protein. (J:231237)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnai3 Mutation: 49 strains or lines available

References

• Original: J:231237 Young SA, et al., CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis. Int J Mol Sci. 2015;16(10):24732-50
• All: 1 reference(s)