Spaar^em1Ppp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spaar^em1Ppp
• Name: small regulatory polypeptide of amino acid response; endonuclease-mediated mutation 1, Pier Paolo Pandolfi
• MGI ID: MGI:6343396
• Synonyms: Spar^-
• Gene: Spaar Location: Chr4:43730045-43732084 bp, + strand Genetic Position: Chr4, 23.05 cM
• Alliance: Spaar^em1Ppp page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A deletion of the initiation ATG codon was generated by CRISPR/Cas9 mutagenesis in order to ablate the polypeptide expression while maintaining the expression of the host lncRNA. Immunoblot analysis confirmed the absence of polypeptide expression in skeletal muscle from homozygous mutant mice. (J:238474)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spaar Mutation: 1 strain or line available

References

• Original: J:238474 Matsumoto A, et al., mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR polypeptide. Nature. 2017 Jan 12;541(7636):228-232
• All: 1 reference(s)