Bcas1^tm1Himo
Targeted Allele Detail

Summary

• Symbol: Bcas1^tm1Himo
• Name: brain enriched myelin associated protein 1; targeted mutation 1, Hisashi Mori
• MGI ID: MGI:6343403
• Synonyms: BCAS1^-
• Gene: Bcas1 Location: Chr2:170188911-170269765 bp, - strand Genetic Position: Chr2, 91.41 cM
• Alliance: Bcas1^tm1Himo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242041
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A point mutation 12 bases downstream of adenine of the start codon was introduced to generate an in-frame stop codon. A mouse phosphoglycerate kinase promoter-driven neomycin resistantance gene (pgkneo) and a prokaryotic em7 promoter-driven zeocin-resistant gene (em7zeo) was inserted downstream of the engineered point mutation. Western blot analysis confirmed the absence of protein expression in the cerebral cortex and sciatic nerve of homozygote mutant mice. (J:242041)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bcas1 Mutation: 40 strains or lines available

References

• Original: J:242041 Ishimoto T, et al., Mice lacking BCAS1, a novel myelin-associated protein, display hypomyelination, schizophrenia-like abnormal behaviors, and upregulation of inflammatory genes in the brain. Glia. 2017 May;65(5):727-739
• All: 1 reference(s)