Tg(Elf5-cre/ERT2,-GFP)2Rcha
Transgene Detail

Summary

• Symbol: Tg(Elf5-cre/ERT2,-GFP)2Rcha
• Name: transgene insertion 2, Rumela Chakrabarti
• MGI ID: MGI:6343502
• Synonyms: Elf5^CreERT2-GFP
• Transgene: Tg(Elf5-cre/ERT2,-GFP)2Rcha Location: unknown
• Alliance: Tg(Elf5-cre/ERT2,-GFP)2Rcha page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Elf5-cre/ERT2,-GFP)2Rcha expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Elf5 (MGI:1335079)

• Mutation details: A partial coding sequence of exon 2 of Elf5 was replaced with CreERT2-P2A-eGFP-SV40pA-Frt-Neo cassette-Frt by homologous recombination into BAC clone RP23-44A14. The Neo cassette flanked by Frt sites was deleted after FLP-mediated recombination. Six lines were generated and three lines with GFP expression in the mammary glands were kept for the initial screening. Line 2 and 5 showed strong GPF and essentially identical cre activity after tamoxifen induction. Line 2, male founder was selected for the detailed analysis. (J:277468)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Elf5 (mouse) Summary of all recombinase alleles driven by Elf5. Comparative matrix view of recombinase activities Alliance mouse Elf5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:277468 Singh S, et al., A new Elf5(Cre) (ERT) (2-) (GFP) BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissues. FEBS Lett. 2019 May;593(10):1030-1039
• All: 1 reference(s)