Mcidas^em1Sroy
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mcidas^em1Sroy
• Name: multiciliate differentiation and DNA synthesis associated cell cycle protein; endonuclease-mediated mutation 1, Sudipto Roy
• MGI ID: MGI:6343523
• Synonyms: Mci^-
• Gene: Mcidas Location: Chr13:113130379-113136928 bp, + strand Genetic Position: Chr13, 63.93 cM
• Alliance: Mcidas^em1Sroy page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology was used to generate a deletion of 32 bp in exon 2. This allele is predicted to encode a severely C-terminally truncated protein, lacking all of the required functional domains. Sequence analysis of cDNA obtained from tracheal tissue of homozygous mutant mice confirmed the 32 bp deletion. RT-qPCR analysis revealed a severe reduction of transcript levels in cultures of airway cells from homozygous mutant mice. (J:274880)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcidas Mutation: 17 strains or lines available

References

• Original: J:274880 Lu H, et al., Mcidas mutant mice reveal a two-step process for the specification and differentiation of multiciliated cells in mammals. Development. 2019 Mar 15;146(6):dev172643
• All: 2 reference(s)