Grem2^tm1Akh
Targeted Allele Detail

Summary

• Symbol: Grem2^tm1Akh
• Name: gremlin 2, DAN family BMP antagonist; targeted mutation 1, Antonios Hatzopoulos
• MGI ID: MGI:6343527
• Synonyms: Grem2^-
• Gene: Grem2 Location: Chr1:174661352-174749386 bp, - strand Genetic Position: Chr1, 81.08 cM, cytoband H3
• Alliance: Grem2^tm1Akh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:250034
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 2 was replaced by a pu(delta)TK (fusion of puromycin and truncated thymidine kinase genes)/EM7neo (kanamycin resistance gene under the synthetic bacterial EM7 promoter) selection cassette via homologous recombination. This resulted in the deletion of the entire coding sequence and most of the 3' untranslated region of the gene, leading to complete loss of the encoded protein. Immunofluorescence analysis revealed that, after myocardial infarction, protein expression was robustly induced in peri-infarct cardiomyocytes of wild-type hearts, whereas protein expression was completely absent in homozygous mutant hearts. (J:250034)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grem2 Mutation: 16 strains or lines available

References

• Original: J:250034 Sanders LN, et al., BMP Antagonist Gremlin 2 Limits Inflammation After Myocardial Infarction. Circ Res. 2016 Jul 22;119(3):434-49
• All: 1 reference(s)