Gm26878^em1Psta
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gm26878^em1Psta
• Name: predicted gene, 26878; endonuclease-mediated mutation 1, Pawel Stankiewicz
• MGI ID: MGI:6343542
• Synonyms: Gm26878^-
• Gene: Gm26878 Location: Chr8:121606742-121607956 bp, + strand Genetic Position: Chr8, Syntenic
• Alliance: Gm26878^em1Psta page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The entire gene was deleted via CRISPR/Cas9 mutagenesis. Sanger sequencing confirmed the presence of a single 2396-bp interval deletion spanning Gm26878 in G1 progeny generated from founder M1. (J:250380)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gm26878 Mutation: 0 strains or lines available

Notes

Although Gm26878 is located in the mouse region syntenic with the human FOXF1 upstream enhancer, RT-qPCR analysis presented in J:250380 did not reveal any significant differences in Foxf1 expression in the lungs of E18.5 homozygous embryos relative to wild-type controls. These data indicate that the regulation of FOXF1 expression differs between human and mouse.

References

• Original: J:250380 Szafranski P, et al., CRISPR/Cas9-mediated deletion of lncRNA Gm26878 in the distant Foxf1 enhancer region. Mamm Genome. 2017 Aug;28(7-8):275-282
• All: 1 reference(s)